Experiment Microbio

PART 2 : ASEPTIC TECHNIQUES AND TRANSFER OF CULTURES Objectives: 1) To apply antiseptic techniques in microbial cultivation 2) To isolate pristine small town by streak case method 3) To assign bacteria by several(prenominal) methods INTRODUCTION Microbes exist everywhere and they become the showtime of taint towards any(prenominal) effected pure cultures. Pure culture is springy in any laboratory which participates in activities related to scientific explore especially in biotechnology. Pure culture, which is defined as a culture containing one particular species of microbe, impart modify scientists to come scientific research and identify the microbe for manakin in diagnostic purposes. Therefore, it is all important(p) for any microbiologists to maintain the ingenuousness of cultures, stop from any contaminant. Techniques that can help microbiologists avoid contamination ar referred to as aseptic techniques. These techniques are also crucial in maintainin g safety for some other(prenominal) workers and environment. Basically, aseptic techniques work by avoiding contacts in the midst of contaminants and pure cultures. Without aseptic techniques, this would allow other microbes to contaminate the pure culture.

In this experiment, the broth culture entrust transfer into liquidity intermediate, cultures from agar medium transfer to semisolid agar medium using stab inoculation and another one is transfer cultures from solid medium to slant agar. We will also establish the pure cultures by streak graduated table method to set out star cell of bacteria. declaration Inoculum sourceTransferred to:Observation(Draw and label), Discussion and conclusions NA,petri salmon! pinkNA,petri dish BrothBroth nutritive agarStab Nutrient agarSlant psyche 1.Explain how procedures in streak plate method would generate single isolated colonies? The streaking have to do carefully to avoid pause of agar medium. The bacteria only(prenominal) be taken once from the clear-cut colony to do the first streak. Bacteria are picked up by sterile...If you motive to get a full essay, pose it on our website: OrderEssay.net

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